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9 cis RA and ATRA, which have the highest affinity for Rxrs and Rars, blocked eye migration most effectively, whereas 13 cis RA, which has low affinity for the receptors, was less effective. P values, as determined by chi square test,https://www.cheapjerseys-nfl.com indicate significant differences between the blind and ocular sides in the different treatments relative to the control. 9 cis RA treatment, n = 83 individuals survived; ATAR treatment, n = 99 individuals survived; 13 cis RA treatment, n = 59 individuals survived; control group with DMSO treatment, n = 70 individuals survived. (c) Representative phenotypes caused by microinjection of RAs. The images show eye symmetry observed in specimens injected with 9 cis RA (blind side) (top left, n = 4) or ATRA (blind side) (middle left, n = 3); eye asymmetry was observed in specimens injected with 13 cis RA (ocular side) (top right, n = 34) or DMSO (ocular side) (control; middle right, n = 44). Scale bars, 1 mm.
Age matched wt siblings served as controls for potential rapamycin side effects. At the time of being killed (clinical complications, or age 52 weeks), blood was collected to measure hematocrit and rapamycin levels. Polyps in the small and large intestine were counted and the spleen was weighed.